NMR laboratory

The NMR service offers specialist support and advice in NMR spectroscopy. This includes:

  • guidance in the choice of experiment
  • help in setting up new experiments
  • training for the technical development of researchers and staff in NMR spectroscopy
  • the opportunity to undertake collaborative research projects requiring NMR expertise

Experiments Available for NMR customers – School of Chemistry.

There are two distinct braches to the NMR provision for researchers in the School of Chemistry . Walk-up service is provided on four robot spectrometers, a wide range of basic experiments is available, with the restriction that daytime experiments are limited to a maximum of 15 minutes per spectrum and overnight/weekend acquisitions may not exceed 3 hours per spectrum. There is a request service for longer or more complex experiments, the spectra for which are recorded by NMR Staff.

Walk-up Service

  • B400  Bruker Avance III 400 MHz room B.12, runs 24/7
    Proton, Carbon, Dept, Cosy, Hsqc, Hmbc, 31P, 19F, Noesy
    Individual groups also have additional experiments enabled
    which are group specific.
  • B300 Bruker Avance 300 MHz room B11, runs 24/7
    Proton, Carbon, Dept, Cosy, Hsqc, Hmbc
  • B500 Bruker Avance II+ 500 MHz, room B11, 10am Mon to 10am Weds
    Proton, Carbon, Dept, Cosy, Hsqc, Hmbc,
  • B200 Bruker Avance 200 MHz, 2nd floor teaching, out of term time
    Proton, 31P

Staff-run Service

  • Proton, Carbon, Dept, Cosy, Hsqc, Hmbc, 31P, 7Li , 11B,other heteronuclei, Noesy, Roesy, Tocsy
  • 1D Noesy(nOe), water suppression inc. 2D expts
  • Variable Temperature, standard range 120 deg C to -40 deg C. depending on solvent
  • Time course studies

For more information on any of the experiments above please speak to any of the staff in the NMR section who will be happy to advise or contact Ralph Adams, and for MIB information Barbara Gore.

Available Spectrometers

This is a list of NMR spectrometers available in the University of Manchester, School of Chemistry.

1. Bruker Avance III new 400MHz spectrometer.

This is our new state-of-art instrument, it is equipped with an auto-tuning multi-nuclear direct observation probe. A 60 postion autosampler is used for loading. It is configured for 24/7 walk-up use by students and  researchers. A wide range of both 1D and 2D proton and carbon experiments is available. 31P ,19F, 11B, 2H and 77Se nuclei are also configured.

2. Bruker Avance 300 MHz spectrometer

This refurbished instrument is equipped with a new gradient shimming facility, and data system. It has a 60 position autosampler and a dual direct observation probe which is optimised for 13C observation experiments. It is used as a 24/7 walk-up facility and supports 1D proton and carbon experiments together with a wide range of proton homo-nuclear, 2D and hetero-nuclear proton-carbon correlation 2D experiments.

3. Bruker Avance 200MHz spectrometer

This instrument has been refurbished, including a new data system and new 60 position autosampler. It has a proton and X -channel tuneable probe, but does not have auto-tune for online nucleus selection. It is used, first line, to support undergraduate projects, but will be available for walk-up use by researchers and postgraduates outside of the periods which are needed for undergraduate use. The main function will be for quick turnaround proton spectra for reaction monitoring. 31P spectra can also be recorded under automation.

4. Varian Inova 300 MHz spectrometer

This is an older instrument with a 50 position autosampler and a dual proton/carbon probe. It is configured for 24/7 walk-up use. It supports a range of 1D and 2D experiments which are broadly similar to those available on the Bruker Avance 300MHz instrument. It does have gradient shimming and gradient assisted 2D experiments. Generally now used only as a backup.

5. Bruker Avance III new 400MHz spectrometer.

This new state of the art spectrometer is configured mainly for NMR staff  to  use for our NMR service for longer or more complex experiments. Users access the facility via  filling in a request in the usual electronic/paper request form. It has a versatile auto-tune probe which will tune to variety of nuclei. Pulsed field gradient enhanced 2D experiments, as well as the usual proton and carbon spectra will be performed. Variable temperature and time course experiments are also catered for.

6. Varian VMS and Bruker Avance II+ 500 MHz spectrometers.

Both these units are virtually state of the art. Both have been set up in the last 12 months. They will support mainly research use, but NMR staff will be able to reserve time on either for running experiments which require higher field, sensitivity or probe requirements than is available on the Avance 400 model. Both these spectrometers are fully equipped for automatic operation with 50(Varian) and 60 (Bruker) autosamplers fitted.Currently the Bruker instrument is available for walk-up use on two days each week as a minimum. Sometimes weekends are also available for walk-up users.

7. Varian Inova 400MHZ and 300 MHz spectrometers.

These two older manually operated spectrometers will continue to be supported for the time being. They will be used as bookable units for both users and NMR staff. A wide range of experiments may be performed, particularly on the 400MHz model. The 300 MHz model is rather more basic and lacks pulsed field gradients.

 

For further information please contact Barbara Gore or Ralph Adams.

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