Professor Gareth Morris and Dr Mathias Nilsson: “Pure shift” HSQC – heteronuclear 2D NMR spectra without multiplet structure".

The NMR methodology group’s recent “top 10%” paper in Angewandte Chemie (DOI: 10.1002/anie.201305709) describes a method that is unusual in simultaneously improving both resolution and sensitivity – increasing one usually means having to accept a reduction in the other.

The resultant experiment suppresses multiplet structure in both dimensions of the spectrum, and has the highest resolution to date for any heteronuclear correlation 2D NMR method. Preliminary results show that the method works well for both 13C-1H and 15N-1H correlation, so it should prove useful both in chemistry and in structural biology. The work was an interdisciplinary and an international collaboration, involving colleagues from MIB, postdocs from Iran, Nepal, Spain and the UK, and a visitor from Hungary (funded by the European Molecular Biology Organisation). The method should be readily adaptable to other types of heteronuclear 2D experiment; for example work is in progress with colleagues in Debrecen and Darmstadt on analogues for use in the NMR of oriented samples.

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